Composite

Part:BBa_K4458005

Designed by: Pavin Rajagopal   Group: iGEM22_East_Coast_BioCrew   (2022-09-10)


pcbA4 assembly

pcbA4 is a reductive dehalogenase present in the Dehalococcoides species. It specializes in the dechlorination of PCBs, or polychlorinated biphenyls. It was identified in the paper "Growth of Dehalococcoides spp. and increased abundance of reductive dehalogenase genes in anaerobic PCB-contaminated sediment microcosms" (Ewald JM, Humes SV, Martinez A, Schnoor JL, Mattes TE. Growth of Dehalococcoides spp. and increased abundance of reductive dehalogenase genes in anaerobic PCB-contaminated sediment microcosms. Environ Sci Pollut Res Int. 2020 Mar;27(9):8846-8858. doi: 10.1007/s11356-019-05571-7. Epub 2019 Jun 17. PMID: 31209752; PMCID: PMC6918016.). It accumulates faster than similar proteins, pcbA1 and pcbA5, it accumulates faster.

We were able to successfully clone pcbA4 into E. coli as shown by the band of ~1500 bp on a colony screening PCR gel.
pcba4-2.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 13
    Illegal XbaI site found at 234
    Illegal PstI site found at 1
    Illegal PstI site found at 131
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1
    Illegal PstI site found at 131
    Illegal NotI site found at 19
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 13
    Illegal XbaI site found at 234
    Illegal PstI site found at 1
    Illegal PstI site found at 131
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 13
    Illegal XbaI site found at 234
    Illegal PstI site found at 1
    Illegal PstI site found at 131
    Illegal AgeI site found at 1430
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1393


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Categories
Parameters
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